A Novel Amperometric Immunosensor for Phytohormone Abscisic Acid Based on In Situ Chemical Reductive Growth of Gold Nanoparticles on Glassy Carbon Electrode

Authors: Wang, Ruozhong¹; Li, Yuwei¹; Li, Qian¹; Shen, Guoli²; Xiao, Langtao¹

Source: Analytical Letters, Volume 42, Number 17, 2009 , pp. 2893-2904(12)

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Abstract:

An amperometric immunosensor for phytohormone abscisic acid was developed based on in situ chemical reductive growth of gold nanoparticles on glassy carbon electrode. First, an approximate 10 nm gold layer was sputtered uniformly onto the electrode surface, and then gold nanoparticles were grown directly on the gold layer for antibody adsorption by immersing the electrode into the H2AuCl4 solution. Determination was based on an enzyme-linked competitive immunoreaction between free and enzyme-labeled abscisic acid to bind on immobilized antibody on electrode. The linear response was from 10 ng/ml to 10 µg/ml with a detection limit of 5 ng/ml.

Keywords: Abscisic acid (ABA); gold nanoparticles; immunoreaction; in situ chemical reduction

Document Type: Research article

DOI: http://dx.doi.org/10.1080/00032710903201909

Affiliations: 1: College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China 2: College of Chemistry and Chemical Engineering, Hunan University, Changsha, China

Publication date: 2009-01-01