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Anti-lipid peroxidation and induction of apoptosis in the erythroleukaemic cell line K562 by extracts from (Tunisian) Rhamnus alaternus L. (Rhamnaceae)

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Abstract:

Total oligomer flavonoids (TOF) enriched and ethyl acetate (EA) extracts from Rhamnus alaternus induce apoptotic death in human chronic myelogenous leukaemia K562 cell line, as demonstrated by gel electrophoresis, which demonstrates the characteristic ladder patterns of DNA fragmentation and the proteolytic cleavage of poly(ADP ribose) polymerase (PARP). The effect of R. alaternus extract in reducing oxidative stress was evaluated by anti-lipid peroxidation which was monitored by measuring malondialdehyde level in K562 cultured cells. The TOF and EA extracts were found to be effective to protect against lipid peroxidation. Their IC50 values were 196 and 273 µg mL−1, respectively. These findings suggest that R. alaternus extracts exhibit potential antioxidant and proapoptotic properties.

Keywords: DNA fragmentation; PARP; Rhamnus alaternus; antioxidant; apoptosis; leukaemia cells; lipid peroxidation

Document Type: Research Article

DOI: https://doi.org/10.1080/14786419.2010.490783

Affiliations: Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03, Faculté de Pharmacie de Monastir, Rue Avicenne5019 Monastir, Tunisie

Publication date: 2011-07-01

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