Genetic tagging of Azotobacter chroococcum for colonization on wheat ( Triticum aestivum ) and cotton ( Gossypium sp .) roots

Authors: Kumar, Rakesh; Bhatia, Ranjana; Sikka, Virendra; Suneja, Sunita; Narula, Neeru

Source: Archives of Agronomy and Soil Science, Volume 52, Number 3, June 2006 , pp. 359-364(6)

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Abstract:

Azotobacter chroococcum strains E12, HT57 were genetically tagged with lac Z, gfp to study the colonization behaviour on wheat ( Triticum aestivum ) and cotton ( Gossypium sp .) in soil under controlled conditions. 10 3 – 10 4 cfu g −1 soil of HT57 lac Z were found to colonize roots of both cotton and wheat crops whereas 1.7 × 10 4 – 7.2 × 10 4 cfu g −1 soil of E12 gfp was colonizing wheat roots and 1.6 × 10 4 – 9.3 × 10 4 cfu g −1 soil of E12 gfp colonized cotton roots respectively. Tagged strains colonized mostly on root tips compared to basal roots in both the crops.

Keywords: Colonization; lac Z; gfp; Azotobacter chroococcum; Triticum aestivum; Gossypium sp

Document Type: Research article

DOI: http://dx.doi.org/10.1080/03650340600641499

Affiliations: 1: Department of Microbiology, CCS Haryana Agricultural University, Hisar, India

Publication date: 2006-06-01

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