On the Use of Nitrocellulose Membranes for Dialysis-mediated Purification of Ancient DNA from Human Bone and Teeth Extracts

Authors: Kiesslich J.¹; Radacher M.¹; Neuhuber F.¹; Meyer H.J.¹; Zeller K.W.²

Source: Ancient Biomolecules, Volume 4, Number 2, 1 January 2002 , pp. 79-87(9)

Publisher: Taylor and Francis Ltd

Abstract:

One of the crucial steps during ancient DNA purification is the removal of inhibitory substances from hard tissue extracts. We present a cheap and easy-to-perform method for the purification of ancient DNA using nitrocellulose membranes in order to remove inhibitory inorganic (EDTA, calcium, etc.) and organic (humic acid, fulvic acids, etc.) compounds from tissue extracts in a single step procedure. Subsequent ethanol precipitation provides purified and concentrated ancient DNA, suitable for multiplex PCR amplification.

Keywords: Ancient DNA; Dialysis; DNA purification; Hard tissue; Human DNA; STR typing

Document Type: Research article

DOI: http://dx.doi.org/10.1080/1358612021000010668

Affiliations: 1: University of Salzburg, Institute of Legal Medicine, Ignaz Harrer-Straße 79, 5020 Salzburg, Austria 2: Österreichisches Forschungszentrum Dürrnberg, Pflegerplatz 5, 5400 Hallein, Austria

Publication date: 2002-01-01

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