OPTIMISATION OF REACTIVE DYE DEGRADATION BY LACCASE USING BOX-BEHNKEN DESIGN

Authors: Cristovao, R. O.; Tavares, A. P. M.; Loureiro, J. M.; Boaventura, R. A. R.; Macedo, E. A.

Source: Environmental Technology, Volume 29, Number 12, December 2008 , pp. 1357-1364(8)

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Abstract:

A three-level Box-Behnken factorial design with three factors and the Response Surface Methodology were used to optimise the colour removal of the reactive textile dye, Colour Index (C.I.) reactive red 180, by commercial laccase. A mathematical model was developed to study the effect of temperature, pH, enzyme concentration and their interactions on the decolourisation. Enzyme concentration and pH as well as their interaction were the principal factors that affected the decolourisation. The dye degradation was independent of temperature. The model estimated that the highest decolourisation (> 92%) was obtained for 27°C, pH 7.5 and 85 U l-1. This predicted value was experimentally validated, obtaining dye colour removal (540 nm) of 93 ± 1.5%.

Keywords: Response surface; laccase mediator system; optimisation; textile dye

Document Type: Research article

DOI: http://dx.doi.org/10.1080/09593330802379615

Affiliations: 1: Laboratory of Separation and Reaction Engineering (LSRE), Departamento de Engenharia Quimica, Faculdade de Engenharia, Universidade do Porto, Porto, Portugal

Publication date: 2008-12-01