Dot-immunogold filtration assay for rapid screening of three fluoroquinolones

Authors: Sui, Jianxin¹; Lin, Hong¹; Cao, Limin¹; Li, Zhenxing¹

Source: Food and Agricultural Immunology, Volume 20, Number 2, June 2009 , pp. 125-137(13)

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Abstract:

A dot-immunogold filtration assay (DIGFA) was described for simultaneous screening of three fluoroquinolones (FQs): enrofloxacin (ENR), ciprofloxacin (CIP) and norfloxacin (NOR). Colloidal gold particles were used as visible labels, and anti-ENR polyclonal antibodies from mice and rabbits were exploited for gold labelling and membrane coating, respectively. Several parameters affecting DIGFA were optimised, and the operation was validated with pure buffer matrix and spiked eel samples, respectively. The detection limits in eel samples were estimated to be about 20 µg kg-1 for ENR and CIP, and 50 µg kg-1 for NOR. With pretreated nitrocellulose membranes, the determination could be completed within 30 minutes, and the results could be read just by the naked eye without the help of any equipment.

Keywords: fluoroquinolones; dot-immunogold filtration assay; screening

Document Type: Research article

DOI: 10.1080/09540100902889936

Affiliations: 1: Food Safety Laboratory, Ocean University of China, Qingdao, P.R. China