IngentaConnect Immunochemical reactivity of soybean -conglycinin subunits

Authors: Petruccelli, Silvana¹; Gabriel Chirdo, Fernando¹; Cristina Añón, María¹

Source: Food and Agricultural Immunology, Volume 16, Number 1, March 2005 , pp. 17-28(12)

Abstract:

The immunochemical reactivity of two polyclonal sera raised against soybean purified αα′ and β subunits was analysed. We show that anti-αα′ serum mainly reacts with αα′ subunits and β′ subunit, but it does not recognize β subunit. In addition, β subunit is not able to compete with αα′ subunit for anti-αα′ serum recognition sites; therefore the main immunogenic region of αα′ subunits is the extension region. Both anti-αα′ and anti-β sera were able to recognize E. coli produced α subunit, consequently glycosylation is apparently not required for antigen-antibody interaction. Anti-β subunit serum shows strong reactivity against soybean produced β-subunit and recombinant α subunit, while it shows weak recognition of soybean purified αα′ subunits. A mutant of α subunit with the N terminal β barrel region and C terminal α helices deleted, failed to be recognized by anti-β serum. For that reason although N and C terminal domains are structurally equivalent, their immunochemical reactivity is different.

Keywords: β-conglycinin; recombinant soybean subunits; immunoreactivity; soybean 7S globulin

Document Type: Research article

DOI: 10.1080/09540100500054727

Affiliations: 1: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Capital Federal, Argentina

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Immunochemical reactivity of soybean β-conglycinin subunits