Factors influencing colonization of cucumber roots by Clonostachys rosea f. catenulata, a biological disease control agent
The biocontrol fungus Clonostachys rosea f. catenulata (Gliocladium catenulatum) strain J1446, commercially available as Prestop® (Verdera Oy, Finland), is an effective antagonist against several root and foliar greenhouse pathogens. The biocontrol agent forms dense networks of
hyphae on plant roots, grows internally in root epidermal cells, and produces hydrolytic enzymes, all of which lead to a reduction in pathogen propagules. An understanding of the environmental and host factors that influence root colonization by C. rosea f. catenulata is important to maximize
disease control efficacy. Cucumber roots grown in nutrient solution in containers were inoculated with conidia of a GUS-transformed strain of C. rosea f. catenulata. Population levels associated with roots over time were assessed by colony-plate counts, GUS staining and enzymatic assays to
determine GUS activity. Variables such as pH, temperature and growing medium were major factors that influenced population levels, while cucumber cultivar, addition of nutrients, and wounding of roots did not appear to significantly affect colonization. Population density of C. rosea f. catenulata
on roots was highest when the nutrient solution was maintained at pH 5, 6, or 7, and at temperatures of 18-22°C. Lowest colonization levels were observed on roots of plants grown in potting mix or in field soil. Measurement of GUS activity provided a slightly more accurate assessment of
root colonization levels compared to colony-plate counts. These results illustrate the optimal environmental conditions which can ensure maximum root colonization by C. rosea f. catenulata and enhance disease control by the biocontrol agent.
Keywords: C. rosea f. catenulata; G. catenulatum; biological control; colonization; environmental factors
Document Type: Research Article
Affiliations: Department of Biological Sciences, 8888 University Dr., Simon Fraser University, Burnaby, British Columbia, Canada
Publication date: 01 January 2010
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