PCR-RFLP assays for discerning three weevil stem feeders (Ceutorhynchus spp.) (Col.: Curculionidae) on garlic mustard (Alliaria petiolata)
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assays were developed to rapidly and cost-effectively differentiate the morphologically indistinguishable larval stages of three species, Ceutorhynchus alliariae, C. roberti and C. scrobicollis, proposed for
the biological control of Alliaria petiolata. A PCR-RFLP using TaqαI restriction enzyme can differentiate the three target species only, while a PCR-RFLP double digestion using the restriction enzymes AluI and TaqαI should enable, based on virtual digestion, distinguishing them
from 12 other Ceutorhynchus species associated with A. petiolata.
Keywords: Alliaria petiolata; Ceutorhynchus; PCR-RFLP; biological control; garlic mustard; natural enemy
Document Type: Research Article
Affiliations: 1: Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO, USA 2: Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO, USA,Graduate Degree Program in Ecology, Colorado State University, Fort Collins, CO, USA
Publication date: 01 October 2009
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