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Viability of Stored Conidia of Metarhizium flavoviride Gams and Rozsypal, Produced under Differing Culture Regimes and Stored with Clays

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Abstract:

Conidia of Metarhizium flavoviride were cultured under a range of conditions and stored , with or without clays and silica gel , as powder or in oil . Fungal biomass was produced in shake flasks in liquid media containing nutrients before being added to sterilized rice for conidial production . Conidia pre - cultured under a low C:N ratio , or dried over a 9 - day period compared with 2 days before being placed in storage , showed greatest loss of viability . Conidia stored at 10 o C survived better than those stored at + 30 o C . A range of clays had no significant effect on storage of dried conidia but montmorillonite K10 clay was often harmful in terms of conidial viability . Conidia survived better when stored as dry powder than when stored in a mixture of mineral and vegetable oils . The addition of silica gel proved beneficial even when dried conidia were being stored .

Keywords: CONIDIA; CULTURING CONDITIONS; METARHIZIUM FLAVOVIRIDE; STORAGE; TEMPERATURE

Document Type: Research Article

DOI: http://dx.doi.org/10.1080/09583159730749

Publication date: September 1, 1997

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