@article {KONG:2005:1058-8337:135, author = "KONG, IN", author = "PARK, T.", author = "AHN, T.", author = "BURLAGE, R.", title = "Bioluminescence Activity and Degradation Kinetics of Genetically Engineered Strain P. putida mt-2 RB 1401 in Soil System with m-Xylene", journal = "Soil and Sediment Contamination (formerly Journal of Soil Contamination)", volume = "14", year = "2005", abstract = "Strain RB1401 contains a plasmid with a fusion between the upper pathway promoter of the TOL plasmid xyl genes and a lux gene cassette. Bioluminescence activity appeared in the early period of biodegradation of m-xylene, and was observed at all tested conditions of temperature (15, 27, 35°C) and pH (5, 7, 9), showing in the range of 300 ~ 6100 RLU. Biokinetics of strain RB1401 in the presence of m-xylene were as follows: Y 0.56 mg/mg, µ max 2.67 day -1 , and K s 19.25 mg/L. A ratio of C:N(KNO 3 ):P(KH 2 PO 4 ) = 100:8:1.5 and 0.01% (w/v) of H 2 O 2 were chosen for optimum biostimulation conditions of RB1401 with m-xylene in soil system. These results demonstrate the capability of such a genetically engineered bacteria for the development of biomonitoring and bioremediation in soil environments contaminated with specific volatile organic compounds.", pages = "135-142(8)", url = "http://www.ingentaconnect.com/content/tandf/bssc/2005/00000014/00000001/art00004" doi = "doi:10.1080/15320380590911760" }