Bioluminescence Activity and Degradation Kinetics of Genetically Engineered Strain P. putida mt-2 RB 1401 in Soil System with m-Xylene
Authors: KONG, IN1; PARK, T.2; AHN, T.3; BURLAGE, R.4
Source: Soil and Sediment Contamination (formerly Journal of Soil Contamination), Volume 14, Number 1, 2005 , pp. 135-142(8)
Publisher: Taylor and Francis Ltd
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Abstract:
Strain RB1401 contains a plasmid with a fusion between the upper pathway promoter of the TOL plasmid xyl genes and a lux gene cassette. Bioluminescence activity appeared in the early period of biodegradation of m-xylene, and was observed at all tested conditions of temperature (15, 27, 35°C) and pH (5, 7, 9), showing in the range of 300 ~ 6100 RLU. Biokinetics of strain RB1401 in the presence of m-xylene were as follows: Y 0.56 mg/mg, µ max 2.67 day -1 , and K s 19.25 mg/L. A ratio of C:N(KNO 3 ):P(KH 2 PO 4 ) = 100:8:1.5 and 0.01% (w/v) of H 2 O 2 were chosen for optimum biostimulation conditions of RB1401 with m-xylene in soil system. These results demonstrate the capability of such a genetically engineered bacteria for the development of biomonitoring and bioremediation in soil environments contaminated with specific volatile organic compounds.Keywords: Biokinetics; bioluminescence activity; lux gene; TOL plasmid
Document Type: Research article
DOI: 10.1080/15320380590911760
Affiliations: 1: Department of Environmental Engineering Yeungnam University Gyungbuk Korea 2: Department of Civil Engineering Hanyang University Seoul Korea 3: Department of Civil and Environmental Engineering Woosong University Daejon Korea 4: Health Sciences Department University of Wisconsin, Milwaukee Gyungbuk WI USA
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