Provider: Ingenta Connect
Database: Ingenta Connect
Content: application/x-research-info-systems
TY - ABST
AU - Willenberg, Bradley Jay
AU - Zheng, Tong
AU - Meng, Fan-Wei
AU - Meneses, Juan Carlos
AU - Rossignol, Candace
AU - Batich, Christopher D.
AU - Terada, Naohiro
AU - Steindler, Dennis A.
AU - Weiss, Michael D.
TI - Gelatinized Copper–Capillary Alginate Gel Functions as an Injectable Tissue Scaffolding System for Stem Cell Transplants
JO - Journal of Biomaterials Science, Polymer Edition
PY - 2011-01-01T00:00:00///
VL - 22
IS - 12
SP - 1621
EP - 1637
KW - STEM CELL
KW - ALGINATE
KW - GELATIN
KW - ANISOTROPY
KW - IONOTROPY
KW - BIOMATERIAL
KW - TISSUE SCAFFOLD
KW - CAPILLARY
KW - TISSUE ENGINEERING
N2 - In severe hypoxic–ischemic brain injury, cellular components such as neurons and astrocytes are injured or destroyed along with the supporting extracellular matrix. This presents a challenge to the field of regenerative medicine since the lack of extracellular matrix and supporting
structures makes the transplant milieu inhospitable to the transplanted cells. A potential solution to this problem is the use of a biomaterial to provide the extracellular components needed to keep cells localized in cystic brain regions, allowing the cells to form connections and repair
lost brain tissue. Ideally, this biomaterial would be combined with stem cells, which have been proven to have therapeutic potentials, and could be delivered via an injection. To study this approach, we derived a hydrogel biomaterial tissue scaffold from oligomeric gelatin and copper–capillary
alginate gel (GCCAG). We then demonstrated that our multipotent astrocytic stem cells (MASCs) could be maintained in GCCAG scaffolds for up to 2 weeks in vitro and that the cells retained their multipotency. We next performed a pilot transplant study in which GCCAG was mixed with MASCs
and injected into the brain of a neonatal rat pup. After a week in vivo, our results showed that: the GCCAG biomaterial did not cause a significant reactive gliosis; viable cells were retained within the injected scaffolds; and some delivered cells migrated into the surrounding brain
tissue. Therefore, GCCAG tissue scaffolds are a promising, novel injectable system for transplantation of stem cells to the brain.
UR - https://www.ingentaconnect.com/content/tandf/bsp/2011/00000022/00000012/art00006
M3 - doi:10.1163/092050610X519453
UR - https://doi.org/10.1163/092050610X519453
ER -