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Active Targeting Behaviors of Biotinylated Pluronic/Poly(Lactic Acid) Nanoparticles In Vitro through Three-Step Biotin–Avidin Interaction

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Abstract:

In order to prepare targeted drug carriers, previously a biotin group has been attached by our group to the end of Pluronic F87/poly(lactic acid) and Pluronic P85/poly(lactic acid) block co-polymers to obtain B-F87–PLA and B-P85–PLA, respectively. In this paper, the active targeting properties of B-F87–PLA and B-P85–PLA nanoparticles in vitro were investigated through a three-step biotin–avidin interaction by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) tests and fluorescence microscopy (FM). Two kinds of human ovarian cancer cells (OVCAR-3 and SKOV-3) and paclitaxel were chosen for the cytotoxicity tests. CA-125 antigen is over-expressed on OVCAR-3 cells but not on SKOV-3 cells. The loading and release behavior of paclitaxel loaded in B-Pluronic–PLA nanoparticles were also studied. Paclitaxel loaded in both B-F87–PLA and B-P85–PLA nanoparticles shows an initial rapid release followed by a slow release period. Compared with SKOV-3 cells, the cytotoxicity results implied that paclitaxel-loaded B-Pluronic–PLA nanoparticles were delivered more effectively to OVCAR-3 cells due to the specific interaction between the biotin groups on the surface of B-Pluronic–PLA nanoparticles and the avidin/biotinylated MAb X306/CA-125 antigen complexes on the surface of OVCAR-3 cells. The active targeting properties of B-F87–PLA nanoparticles were further confirmed by FM.

Keywords: BIOTIN-PLURONIC–POLY(LACTIC ACID); CO-POLYMER; NANOPARTICLES; PACLITAXEL; TARGETED DRUG DELIVERY

Document Type: Research Article

DOI: http://dx.doi.org/10.1163/092050610X519444

Affiliations: 1: School of Life Science, Jiangxi Science & Technology Normal University, Nanchang 330013, P. R. China., Email: xyxiong@gmail.com 2: School of Life Science, Jiangxi Science & Technology Normal University, Nanchang 330013, P. R. China

Publication date: January 1, 2011

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