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Bone Regeneration by Lactoferrin Released from a Gelatin Hydrogel

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Abstract:

The objective of this study is to evaluate the potential of lactoferrin (LF), an iron-binding glycoprotein, to induce bone regeneration. A biodegradable gelatin hydrogel was prepared to allow LF release in vivo in a sustained fashion. When subcutaneously implanted into the back of mice, the gelatin hydrogel incorporating LF showed a longer LF retention period at the site of implantation than that of LF solution injection. An in vitro culture experiment with 3T3E1 cells (mouse-derived osteoblasts) revealed that the cells were proliferated to a significantly greater extent by the repeated addition of LF compared with a single addition of LF at the same dose. Following the implantation of gelatin hydrogels incorporating LF into a skull bone defect of rats, a significantly stronger bone regeneration at the defect was observed than in LF-free- or low-LF-treated rats. It is concluded that the sustained release from the gelatin hydrogels enables LF to enhance the in vivo activity of bone regeneration.

Keywords: BONE REGENERATION; LACTOFERRIN; SUSTAINED RELEASE

Document Type: Research Article

DOI: http://dx.doi.org/10.1163/092050610X517095

Affiliations: 1: Institute for Frontier Medical Sciences, Kyoto University, 53 Kawara-cho Shogoin, Sakyo-ku, Kyoto 606-8507, Japan; Department of Veterinary Internal Medicine, Faculty of Agriculture, Tottori University, Minami 4-101, Koyama-cho, Tottori 680-8553, Japan 2: Department of Veterinary Internal Medicine, Faculty of Agriculture, Tottori University, Minami 4-101, Koyama-cho, Tottori 680-8553, Japan 3: Institute for Frontier Medical Sciences, Kyoto University, 53 Kawara-cho Shogoin, Sakyo-ku, Kyoto 606-8507, Japan 4: Institute for Frontier Medical Sciences, Kyoto University, 53 Kawara-cho Shogoin, Sakyo-ku, Kyoto 606-8507, Japan., Email: Japanyasuhiko@frontier.kyoto-u.ac.jp

Publication date: May 1, 2011

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