Preparation of Interconnected Porous Chitosan Scaffolds by Sodium Acetate Particulate Leaching
For tissue-engineering applications, a 3D porous chitosan scaffold was simply prepared from a mixture of acidic chitosan solution and sodium acetate particles as the porogen by a salt-leaching method. Differences in the porous structure in terms of pore morphology and interconnectivity
between the salt-leached chitosan scaffold and phase-separated scaffold as the control were examined by using scanning electron microscopy, protein release and enzymatic degradation tests. A fibroblast (NIH-3T3) cell culture was performed for cell affinity evaluation. The chitosan scaffold
prepared by salt-leaching showed good interconnectivity and improved mechanical properties. Furthermore, the chitosan scaffolds showed a high initial cell adhesion after 4 h cell culture and increased cell proliferation than the control. Thus, salt-leached chitosan scaffolds can be used for
various tissue-engineering applications.
Keywords: CHITOSAN SCAFFOLD; POROUS STRUCTURE; SALT-LEACHING; TISSUE ENGINEERING
Document Type: Research Article
Affiliations: 1: National Core Research Center for Nanomedical Technology, Yonsei University, 262 Seongsanno, Seodaemun-gu, Seoul 120-749, South Korea 2: Denforus Co, 3001-2 Bangbae-dong, Seocho-Gu, Seoul 137-974, South Korea 3: Department of Microbiology, College of Medicine, Yonsei University, Seoul 120-752, South Korea 4:
Publication date: 01 January 2011
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