Biocompatible collagen scaffolds from a human amniotic membrane: physicochemical and in vitro culture characteristics
A reconstituted collagen membrane from human amnion has been investigated as a source of collagen matrix, which could be used as a substratum for culturing human fibroblasts. The suitability of pepsin-solubilized
reconstituted human amniotic membrane, before and after cross-linking with chitosan, as a dermal matrix for culturing fibroblast was assessed by morphologic, physicochemical, cytotoxic and histochemical
methods. Measurement of thermodynamic behaviour, by differential scanning calorimetric (DSC) and thermogravimetric analysis (TGA), and tensile strength suggested that the cross-linked membrane had sufficient
elasticity to serve as an efficient dermal substrate for in vitro culture of fibroblasts. Fibroblasts cultured on the chitosan cross-linked collagen membrane had good adherence, retaining their morphology
as indicated by microscopic analysis. Proliferation of fibroblasts, observed on this membrane affirms its non-toxic nature. These results support the application of reconstituted human amniotic collagen
membrane as collagenous scaffolds to culture fibroblasts in vitro.