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Induced production of rabbit articular cartilage-derived chondrocyte collagen II on polyhydroxyalkanoate blends

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Abstract:

Three-dimensional porous scaffolds made from blend polyesters of polyhydroxyalkanoates (PHA) were evaluated with respect to their effects on collagen II production. Semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to assay collagen II mRNA for evaluation of the ability for the blend scaffolds to induce collagen II production. It was found that scaffold of poly-3-hydroxybutyrate (PHB) blended with a copolyester consisting of 3-hydoxybutyrate-co3-hydroxyhexanate (PHBHHx) in a radio of 1 : 2 had strong ability to induce collagen II production compared with other ratios of PHB/PHBHHx and poly-L-lactic acid (PLA). Collagen II expression level remained approx. 1.5-times higher on the above-mentioned blend compared with that of PLA throughout four weeks cultivation. At the same time, the PHB/PHBHHx blend (1 : 2) exhibited a similar tendency as PLA to maintain the collagen II expression level over the entire culture period. The results showed that the PHB/PHBHHx blend (1 : 2) reported to improve mechanical properties compared with PHB or PHBHHx improved biocompatibility in comparison with PHB, PHBHHx, PLA and other blending systems consisting of PHB/PHBHHX in ratios of 2 :1 and 1 :1, respectively.

Keywords: ARTICULAR CARTILAGE CHONDROCYTE; COLLAGEN II; POLY 3-HYDROXYBUTYRATE-CO-3-HYDROXYHEXANOATE; POLYHYDROXYALKANOATES; POLYHYDROXYBUTYRATE; RT-PCR.

Document Type: Research Article

DOI: http://dx.doi.org/10.1163/156856203322274888

Publication date: July 1, 2003

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