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Fibronectin adsorption or/and covalent grafting on chemically modified PEEK film surfaces

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Abstract:

Poly(ether ether ketone) (PEEK) films were chemically modified, by surface wet chemistry, into PEEK-OH, PEEK-NH2, and PEEK-NCO. Fibronectin (FN) adsorption, in the presence or absence of two non-ionic surfactants, was compared onto PEEK, PEEK-OH, and PEEK-NH2 on which the protein can only be adsorbed, and onto PEEK-NCO on which FN could be covalently grafted. The amounts of FN present on the various supports were assayed by ELISA and LSC (with 125I-labeled FN). The remarkable effect of Pluronic F68 in preventing non-specific protein adhesion on the less hydrophilic surfaces was pointed out. Accordingly, a procedure could be proposed that allows minimal FN adhesion vs FN fixation on PEEK-NCO. The resulting PEEK-FN film, which immobilized 120-150 ng FN cm-2, constitutes a new substratum for cell cultivation.

Keywords: PEEK film; XPS; adsorption; biocompatibilization; cell culture substrata; fibronectin; grafting; surface modification

Document Type: Research Article

DOI: https://doi.org/10.1163/156856299X00865

Affiliations: 1: Laboratoire de Chimie Organique de Synthèse, Université Catholique de Louvain, Département de Chimie, Bâtiment Lavoisier, Place Louis Pasteur; 1348 Louvain-la-Neuve, Belgium, Laboratoire de Biochimie Cellulaire, Université Catholique de Louvain, Département de Chimie, Bâtiment Lavoisier, Place Louis Pasteur 1348 Louvain-la-Neuve, Belgium 2: Laboratoire de Biochimie Cellulaire, Université Catholique de Louvain, Département de Chimie, Bâtiment Lavoisier, Place Louis Pasteur 1348 Louvain-la-Neuve, Belgium 3: Laboratoire de Chimie Organique de Synthèse, Université Catholique de Louvain, Département de Chimie, Bâtiment Lavoisier, Place Louis Pasteur; 1348 Louvain-la-Neuve, Belgium

Publication date: 1999-01-01

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