Cultivation of fibroblast cells on keratin-coated substrata
By means of a cell culture method, the attachment and growth of mouse L929 fibroblast cells were studied on matrices of the (-SDS)- and (+SDS)-keratins, which were extracted from wool in the absence and presence of sodium dodecyl sulfate, respectively. The (+SDS)-keratin showed some
toxic effect on the cell growth, but upon washing with a pH 7/phosphate buffer, the protein behaved similarly to a substratum of the (-SDS)-keratin. The comparative culture assay on the keratins, collagen (type I), and glass revealed that the keratins were more adhesive to the cells and more
supportive for cell proliferation than the collagen and glass. The results were explained by an enhanced initial adsorption of mediator proteins from fetal bovine serum onto the keratin substrata.
Keywords: Keratin; biomaterial; cell attachment; cell-substratum; fibroblast cell
Document Type: Research Article
Affiliations: Department of Bioapplied Chemistry, Faculty of Engineering, Osaka City University, Sumiyoshi-ku, Osaka 558, Japan
Publication date: 01 January 1998
- Access Key
- Free content
- Partial Free content
- New content
- Open access content
- Partial Open access content
- Subscribed content
- Partial Subscribed content
- Free trial content