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Synthesis and characterization of a soluble, temperature-sensitive polymer-conjugated enzyme

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The enzyme, alkaline phosphatase, has been conjugated to a temperature-sensitive polymer which exhibits a lower critical solution temperature (LCST). A series of copolymers containing different molar ratios of N-isopropylacrylamide(NIPAAm) and N-acryloxysuccinimide(NAS) were synthesized and then conjugated to the enzyme. These polymer-enzyme conjugates precipitate and flocculate in aqueous solution above the LCST, and redissolve when cooled below that temperature. The kinetics of the conjugated enzymes have been characterized as a function of temperature and compared to free enzyme. The effect of the conjugation degree between polymer and enzyme on the activity of the conjugated enzymes was also investigated.

Keywords: Conjugated; alkaline phosphatase; enzyme

Document Type: Research Article


Affiliations: Center for Bioengineering, FL-20, University of Washington, Seattle, WA 98195, USA

Publication date: January 1, 1993


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