A wettability gradient as a tool to study protein adsorption and cell adhesion on polymer surfaces

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Abstract:

A new method for preparing a wettability gradient on polymer surfaces was developed. Low density polyethylene sheets were treated in air with corona from a knife-type electrode whose power gradually increases along the sample length. The polymer surfaces oxidized gradually with the increasing power and the wettability gradient was created on the surfaces as evidenced by the measurement of water contact angles, Fourier-transform infrared spectroscopy in the attenuated total reflectance mode, and electron spectroscopy for chemical analysis. The wettability gradient surfaces prepared were used to investigate the interactions of model protein and cells in terms of the surface hydrophilicity/ hydrophobicity of polymeric materials.

Keywords: Chinese hamster ovary cells; Wettability gradients; albumin; cell adhesion; low density polyethylene; protein adsorption

Document Type: Research Article

DOI: http://dx.doi.org/10.1163/156856293X00131

Affiliations: Biomaterials Laboratory, Korea Research Institute of Chemical Technology, P.O. Box 9, Daedeog Danji, Daejeon 305-606, Korea

Publication date: January 1, 1993

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