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RF-plasma-modified polystyrene surfaces for studying complement activation

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Five different plasma modified surfaces were made for studying different aspects of biocompatibility. These surfaces were: 1,2-diaminocyclohexane (DACH), acrylic acid (AA), Hydroxyethylmethacrylate (HEMA), methane and hexamethylene-disiloxane (HMDSO). In addition a polyethylene-glycol (PEG) was made by grafting aldehyde functional PEG to the DACH surface. PEG and HMDSO which are the most hydrophilic and the most hydrophobic surface shows the lowest amount of adsorbed protein of the three proteins studied here (albumin, IgG and C3). Methane, HMDSO and HEMA was found to activate via the classical (complement activation) pathway while the others activated via the alternative pathway.

Keywords: HEMA; HMDSO; Monomer; bio-compatibility; methane

Document Type: Research Article

DOI: http://dx.doi.org/10.1163/156856292X00268

Affiliations: 1: Institute for Surface Chemistry, P.O. Box 5607, S-114 86 Stockholm, Sweden 2: University Hospital Uppsala, Sweden

Publication date: January 1, 1993

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