Study of Ozonated Sunflower Oil Using 1 H NMR and Microbiological Analysis

Authors: Díaz, Maritza¹; Gavín, José²; Gómez, Magali¹; Curtielles, Vicente¹; Hernández, Frank¹

Source: Ozone: Science and Engineering, Volume 28, Number 1, February 2006 , pp. 59-63(5)

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Abstract:

Prior studies have proven that ozonated vegetable oils present a high germicidal power. Ozonation of sunflower oil at different applied ozone dosage was carried out and peroxide and aldehydes indices along with antimicrobial activity were determined. The reaction products were identified using Proton Nuclear Magnetic Resonance Spectroscopy ( 1 H NMR). The principal signals intensity values were used for following the reaction course between ozone and sunflower oil. The reaction was following up to peroxide index values of 1202 mmol-equi/kg. The intensities of olefinic proton signals decreased with the gradual increase in ozone concentration but without disappearing completely. The Criegee ozonides obtained at 107.1 mg/g of ozone doses were approximately 3.9-fold higher than that at beginning of the reaction. The aldehyde protons were observed as a weak intensity signal in all the spectra. The signals belonging to olefinic protons from hydroperoxides appeared weak and increased with the increase in ozone doses. Signals from other oxygenated groups were assigned. The highest action spectrum of antimicrobial activity was obtained with the higher peroxide index. It was concluded that at higher applied ozone doses, the higher the antimicrobial activity potential of ozonized sunflower oil

Keywords: Ozone; Ozonides; 1 H NMR; Sunflower Oil; Antimicrobial Activity

Document Type: Research article

DOI: http://dx.doi.org/10.1080/01919510500479239

Affiliations: 1: Department of Ozonized Substances, Ozone Research Center, National Center for Scientific Research, Havana, Cuba 2: University of the Laguna, Tenerife, Spain

Publication date: 2006-02-01