Ischemic and Ozone Oxidative Preconditioning in the Protection Against Hepatic Ischemic-Reperfusion Injury

Authors: Hussam Hassan Ajamieh¹; Silvia Menéndez²; Nelson Merino¹; Gregorio Martínez-sánchez¹; L. Re³; Olga S. León¹

Source: Ozone: Science and Engineering, Volume 25, Number 3, June 2003 , pp. 241-250(10)

Publisher: Taylor and Francis Ltd

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Abstract:

It has been demonstrated that ozone, probably through a mechanism of oxidative preconditioning (OP), protected the liver against the damage mediated by reactive oxygen species. Taking into account that iscehmic preconditioning (IschP) is also a protective mechanism, a comparative study between both preconditioning settings was performed in order to study the effectiveness of both protective procedures. Rats were randomly divided into 4 groups: 1- control, sham operated (anesthesia and laparotomy plus surgical manipulation); 2- I/R (ischemia for 90 min followed by 90 min reperfusion); 3- Ischp+I/R, as group 2 but submitted to a previous ischemic preconditioning (ischemia 10 min and reperfusion 10 min); 4- OzoneOP+I/R, as group 2 but submitted to a pervious oxidative preconditioning with 15 sessions, daily, of ozone by rectal administration (dose of 1 mg/kg). The comparison between both preconditionings showed no biochemical differences for the parameters evaluated. Nevertheless, the histological study demonstrated that the protective effect produced by the OzoneOP is superior to that achieved with the IschP.

Keywords: Ozone; Medical Applications; Ozone Therapy; Liver Ischemia/Reperfusion

Document Type: Research article

DOI: http://dx.doi.org/10.1080/01919510390481568

Affiliations: 1: Center of Research and Biological Evaluations. Food Pharmacy Institute. Havana University. 2: Ozone Research Center. POBox 6412. Havana city. Cuba. 3: Institute of Experimental and Clinical Medicine, Laboratory of Pharmacology, Univ. Ancona, Via Ranieri 2 60131 Ancona, Italy

Publication date: 2003-06-01