Pyrene Biodegradation by Bacillus spp. Isolated from Coal Tar–Contaminated Soil
Aerobic, mesophilic bacteria from coal tar–contaminated soil were analyzed for pyrene utilization capacity and identified by 16S ribosomal DNA sequencing as members of three genera: Bacillus spp., Pseudomonas sp., and Rhodococcus sp. The soil contained nine
different hazardous polyaromatic hydrocarbons (PAHs): benzo[g, h, i]perylene, dibenzo[a, h]anthracene, indeno[1,2,3-c,d]pyrene, pyrene, acenaphthylene, fluorene, phenanthrene, benzo[k]fluoranthene, and benzo[b]fluoranthene. Bacillus
spp. (PK-6) MTCC 1005 showed 56.4% utilization of pyrene (C16H10) (50 μg ml−1) in 4 days, with growth associated biosurfactant activity and resulted in the formation of five new intermediates: phenanthrene (C14H10), 9,10-diphenylphenanthrene
(C26H18), 9-methoxyphenanthrene (C15H12O), 5,6,7,8-tetrahydro-1-naphthoic acid (C11H12O2), and 1,6,7-trimethylnaphthalene (C13H14). The results suggested that Bacillus spp. could be found
suitable for practical field application for effective in situ PAH bioremediation.
Keywords: 16S rDNA; Bacillus; coal tar-contaminated soil; pyrene
Document Type: Research Article
Affiliations: 1: Department of Biotechnology and Environmental Sciences,Thapar University, PatialaPunjab, India 2: Department of Biotechnology and Bioinformatics,NIIT University, NeemranaRajasthan, India
Publication date: 28 February 2011
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