Technical Note: Degradation of Phenanthrene and Anthracene by Pseudomonas Strain, Isolated From Coastal Area

Authors: Pathak, Hardik¹; Jain, Pankaj¹; Jaroli, D. P.²; Lowry, Madan²

Source: Bioremediation Journal, Volume 12, Number 2, April 2008 , pp. 111-116(6)

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Abstract:

A bacterial strain was isolated from a Mumbai coastal area. It was dosed with anthracene and phenanthrene, and, after 14 days of incubation, it had degraded 90% and 93% of the anthracene and phenanthrene, respectively. The metabolites were extracted and identified by ultraviolet (UV)-visible light absorption, high-performance liquid chromatography, mass spectrometry, and by comparing with actual compounds and data. Neutral extracts from anthracene showed four metabolites, viz 1,2-dihydroxyanthracene, 6,7-benzocoumarin, 1-methoxy-2-hydroxyanthracene, and 9,10 anthraquinone. When Pseudomonas were grown in the presence of phenanthrene, two metabolites, viz 9,10-dihydroxyphenanthrene and 3,4-dihydroxyphenanthrene were identified.

Keywords: anthracene; phenanthrene; UV-visible light absorption; HPLC; mass spectroscopy; Pseudomonas

Document Type: Research article

DOI: http://dx.doi.org/10.1080/10889860802060485

Affiliations: 1: Department of Biotechnology, Mahatma Gandhi Institute of Applied Sciences, Jaipur, India 2: Department of Zoology, University of Rajasthan, Jaipur, India

Publication date: 2008-04-01