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Electron spin resonance. Part one: A diagnostic method in the biomedical sciences

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A review is presented of some of the ways in which electron spin resonance (ESR) spectroscopy may be useful to investigate systems of relevance to the biomedical sciences. Specifically considered are: spin-trapping in biological media; the determination of antioxidant efficiencies; lipid-peroxidation; the use of nitroxides as probes of metabolic activity in cells and as structural probes of cell-membranes; ESR coupled with materials for radiation-dosimetry; food- and drug-irradiation; studies of enzyme systems and of cyclodextrins; diagnosis of cancer and rheumatoid arthritis; measurement of oxidative stress in synovial tissue in preparation for joint replacement; determination of oxidative species during kidney dialysis; measurement of biological oxygen concentrations (oximetry); trapping in living cells of the endothelium-derived relaxing factor nitric oxide (NO); measurement of hydrogen peroxide; determination of drugs of abuse (opiates); ESR measurements of whole blood and as a means to determine the age of bloodstains for forensic analysis are surveyed, and also a determination of the aqueous volume of human sperm cells is described, among other topics.
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Keywords: ANTIOXIDANT EFFICIENCIES; BIOLOGICAL FLUIDS; DIAGNOSIS OF CANCER AND RHEUMATOID ARTHRITIS; ELECTRON SPIN RESONANCE; LIPID-PEROXIDATION; STRUCTURAL PROBE OF CELL-MEMBRANES

Document Type: Research Article

Publication date: 2011-03-01

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