Skip to main content

Free Content An introduction to RNA-mediated gene silencing

Download Article:
(PDF 388.83203125 kb)
Careful analysis of cases where introduction of additional copies of endogenous genes caused coordinate silencing of both the transgene and the endogenous gene laid the ground work for the discovery of RNA-mediated silencing. Silencing begins with the expression and recognition of double-stranded RNA, which is cleaved into short RNAs that recognize, by complementarity, sequences that are targets for down regulation. An RNA target can be regarded (post-transcriptional gene silencing), but the small RNAs can also direct the sequence-specific modification of DNA and chromatin. RNA-mediated gene silencing in eukaryotes may have originated as surveillance mechanism to protect the organism from transposable elements and viruses and then evolved to specify chromosomal modifications and to regulate expression of a significant fraction of endogenous genes by microRNAs. This review seeks to furnish the student and non-expert with some idea of how RNA-mediated silencing was discovered and a broad overview of the present state of knowledge.
No References for this article.
No Supplementary Data.
No Article Media
No Metrics

Keywords: RISC; RNA interference; RNA-mediated gene silencing; co-supression; dicer; gene silencing; microRNAs

Document Type: Research Article

Affiliations: National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 2 Democracy Plaza, Rm 642B, 6707 Democracy Boulevard, MSC 5458, Bethesda, MD 20892-5458, USA

Publication date: 2005-02-15

More about this publication?
  • SCIENCE PROGRESS has for over 100 years been a highly regarded review publication in science, technology and medicine. Its objective is to excite the readers' interest in areas with which they may not be fully familiar but which could facilitate their interest, or even activity, in a cognate field. Science Progress commissions world authorities to contribute articles on the most interesting, important and meaningful topics - ranging from cosmology to the environment - and ensures that they are presented for the most effective use of those in both academia and industry.

    Truly, Science Progress publishes an eclectic mix of articles that no library can afford to be without.

    Volume 100 Issue 4 cover: Tree rings. The historical dating of medieval buildings by dendrochronology and 14C dating is discussed in the article on pages 374–399. Credit: Alexey Borodin/

  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more