Authors: Chen, Fusheng; Zhao, Xiaoyan; Ao, Qiang; Guosheng, Gai; Xue, Wentong; Li, Lite

Source: Chemical Speciation and Bioavailability, Volume 20, Number 3, August 2008 , pp. 191-197(7)

Publisher: Science Reviews 2000 Ltd

Abstract:

The antimicrobial activity of different reverse micelles on microorganisms is been compared using the disc diffusion method. The bis (2-ethylhexyl) sodium sulfosuccinate (AOT) reverse micelle showed a more significant inhibitory effect than do other reverse. micelles, and it had an antimicrobial activity against a broad range of microorganisms. Results from an antimicrobial activity test of isooctane and a forward extraction containing soybean protein suggest that the surfactant was chiefly responsible for inhibiting microbes in AOT/isooctane reverse micelle, while isooctane hardly inhibited the microbial growth. The properties of S. aureus, cultured in the TSB with AOT reverse micellar solution, were identified by the SEM and SDS-PAGE fingerprinting of cell-wall proteins. It is concluded that the cell-wall of the S. aureus decreased in the TSB with AOT reverse micellar solution, and some cell protein subunits of the S. aureus did not occurr, especially between 14.4 and 42.7 kDa, while one new protein subunit at near 97.4 kDa occurred.

Keywords: AOT; REVERSE MICELLE; FORWARD EXTRACTION; ANTIMICROBIAL ACTIVITY; SDS-PAGE FINGERPRINTING; SCANNING ELECTRON MICROSCOPY

Document Type: Research article

DOI: http://dx.doi.org/10.3184/095422908X347287

Publication date: 2008-08-01

More about this publication?

• Chemical Speciation & Bioavailability covers a rapidly expanding area in environmental science.

  Research on the interactions between the chemical forms and behaviour of toxic compounds and their subsequent biological uptake, metabolism and ecological fate involves many scientific fields. These studies are often published in discipline-specific journals, leading to inadequate review and information scatter. This situation hinders both the development of an international community of experienced colleagues and the open flow of information and discussion. Additionally, the importance of speciation and bioavailability research to the development of pollution law and control technologies is being increasingly appreciated by environmental regulatory agencies throughout the world.

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