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Home / Spectroscopy and Spectral Analysis, Volume 33, Number 6

Sensitive Determination of Bovine Serum Albumin by Flow-Injection Chemiluminescence

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A new, rapid and sensitive method for the determination of bovine serum albumin (BSA) by flow-injection coupled with chemiLuminescence detection was establish, which based on BSA enhancing effect on weak chemiLuminescence system of Luminol-H2O2 in alkaline solution. Under the optimized conditions, linearity for BSA was found in the range of 2.5×10-91.0×10-6 mol·L-1, with the detection limit of 5.8×10-10 mol·L-1. And the sampling detecting rate was about 112 samples·h-1. The relative standard deviation(RSD)was found to be 0.8% for 11 replicate determinations of 1.0×10-7 mol·L-1 BSA. The proposed method has been satisfactorily applied to the determination of BSA in the bovine serum samples. The possible mechanism of Luminol-H2O2-BSA was studied by chemiLuminescence spectrum and UV-visible spectrum.

Keywords: Bovine serum albumin; ChemiLuminescence; Flow-injection; Mechanism

Document Type: Research Article

Publication date: 15 June 2013

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  • Spectroscopy and Spectral Analysis, founded in 1981, is sponsored by the Chinese Central Iron & Steel Research Institute. "Spectroscopy and Spectral Analysis" has been indexed in SCI(1999), Ei(1992), MEDLINE(1999), and AJ (1999). "Spectroscopy and Spectral Analysis" publishes original contributions on various fields in Spectroscopy, including research results on laser spectroscopy, IR, Ramn, UV/Vis, Optical Emission, Absorption and Fluorescence spectroscopy, X-ray Fluorescence, and Spectrochemical Analysis, as well as Reseach paper, Research notes, Experimental Technique and Instrument, Review and Progress on the latest development of spectroscopy and spectrochemical anlysis, etc. "Spectroscopy and Spectral Analysis" is published monthly by Peking University Press with book sizes of large 16-mo format , and 292 pages per issue.
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