FTIR Analysis of the Impact of Covalent Coupling on the Secondary Structure of Antibody Protein
The immunolatex was prepared by covalent coupling. FTIR technology combined with substractive spectroscopy, deconvolution, derivation and curve-fitting methods were used to study the structure of the antibody protein on the immunolatex. The result demonstrates that the α-helix
strcture of antibody increases with the increase in the pH value and the concentration of latex. So it is concluded that covalent coupling has a great impact on the secondary structure of antibody protein.
Keywords: Antibody protein; Covalent coupling; Fourier transform infrared(FTIR); Immunolatex; Secondary structure
Document Type: Research Article
Publication date: 01 March 2012
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