Determination of Serum Copper and Zinc in Different Chemical Forms by Graphite Furnace Atomic Absorption Spectrometry with Ethanol-EDTA Precipitation Method
A simple and reliable method was developed for the determination of serum copper and zinc in different chemical forms by graphite furnace atomic absorption spectrometry (GFAAS) with ethanol-EDTA precipitation. The serum and ethanol were mixed with volume ratio 1:2. The mixture
was incubated at 70 and ultra-centrifuged to precipitate proteins. Zinc and copper can be released from albumin after EDTA treating. Thus, macroglobulin-zinc, ceruloplasmin-copper, and albumin-bound zinc or copper could be determined by two proposed precipitation steps. The determination limit
of copper (3σ) was 1.2 μg·L-1 and the recovery was 92.3%-104%, while the determination limit of zinc (3σ) was 0.098 μg·L-1, and the recovery was 90%-107%. This two-step precipitation method can be used to determine serum zinc
and copper in various chemical forms in tumor, Wilson patients and heathy human.
Keywords: Copper; GFAAS; Protein precipitation; Zinc
Document Type: Research Article
Publication date: 01 March 2010
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