Tribulus terrestris (T. terrestris) has been used as a traditional medicine for the treatment of a variety of diseases, including inflammation, edema and hypertension. The aqueous and ethanol extracts of T. terrestris contain alkaloids, flavonoids, tannins, quinines and phenolic
compounds. Tribulusamide D is a compound that has been isolated from the ethanol extract of T. terrestris. The present study investigated the antiinflammatory effect of tribulusamide D on lipopolysaccharide (LPS)stimulated RAW 264.7 macrophages. Tribulusamide D inhibited the production of
LPSinduced nitric oxide and prostaglandin E2, by reducing the expression of inducible nitric oxide synthase and cyclooxygenase2 expression, respectively. The expression of these genes associated with inflammation was determined using reverse transcriptionpolymerase chain reaction and western
blot analysis. Furthermore, tribulusamide D reduced the expression of LPSinduced inflammatory cytokines, including interleukin (IL)6, IL10 and tumor necrosis factorα. They were quantified using an enzymelinked immunosorbent assay. In addition, the present study confirmed that the inhibitory
effects of tribulusamide D on the inflammatory response were mediated through inactivation of mitogenactivated protein kinase p38 and inhibition of nuclear localization of nuclear factorB, which were also determined by western blot analysis. To the best of our knowledge, the current study
is the first to demonstrate that tribulusamide D exerts antiinflammatory activity by altering the expression of inflammatory mediators and cytokines, indicating that tribulusamide D could be developed as a potential therapeutic agent for the treatment of inflammatory disorders.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
Document Type: Research Article
Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, Chungcheongnam 31116, Republic of Korea
Biocenter, Gyeonggi Institute of Science & Technology Promotion, Suwon, Gyeonggi 443270, Republic of Korea
Publication date: 01 October 2017
More about this publication?
Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
- Editorial Board
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Information for Advertisers
- Terms & Conditions
- Ingenta Connect is not responsible for the content or availability of external websites