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Saccharification of Sugarcane Bagasse Using an Enzymatic Extract Produced by Aspergillus fumigatus

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This study investigates the efficiency of a crude enzymatic extract produced by Aspergillus fumigatus SCBM6 by solid state fermentation (SSF) in the hydrolysis of alkali pretreated sugarcane bagasse (PTB). After SSF using in natura sugarcane bagasse (SCB), the enzymatic extract presented 21.33 U.g–1 of β-glucosidase and 544.46 U.g–1 of xylanase. The alkaline pretreatment with sodium hydroxide (2% NaOH (w/v) removed 43% of the lignin from PTB and the cellulosic fraction increased to 75%. The hydrolysis was optimized as a function of time, temperature, and concentration of PTB. After hydrolysis, the maximum yield (30.05%) of total released reducing sugars (TRS) was obtained under the following conditions: 24 h, 55 °C, 2% of PTB and 3 U.g–1 of β-glucosidase (CBU). Furthermore, an approximate TRS value (26.4%) was also obtained after saccharification carried out during 6 h, 55 °C, 4% of PTB and 1 CBU. These results indicate that hydrolysis can be performed in a short incubation period and with low enzymatic load for reasonable TRS release.
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Keywords: A. FUMIGATUS; ALKALINE PRETREATMENT; ENZYMATIC HYDROLYSIS; SOLID STATE FERMENTATION; SUGARCANE BAGASSE

Document Type: Research Article

Publication date: 01 March 2018

This article was made available online on 05 September 2017 as a Fast Track article with title: "Saccharification of Sugarcane Bagasse Using an Enzymatic Extract Produced by Aspergillus fumigatus".

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