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Introducing the concept and application of vitrification for the cryo-conservation of algae – a mini-review

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This review pertains to a project (COBRA QLRT-2000-01645, http://www. cobra. ac. uk) supported by the European Commission's Fifth Framework Programme for Quality of Life and Management of Living Resources. The aim of this initiative is to develop and apply novel cryopreservation protocols to microalgae and cyanobacteria that are difficult to cryopreserve. One such approach utilizes cryoprotective vitrification for the conservation of algal culture collections at -196°C in liquid nitrogen. Achieving a vitrified state depends on increasing cellular viscosity to a critical point at which water forms an amorphous, non-crystalline glass on cooling to ultra-low temperatures. Vitrification has been utilized extensively in medical cryobiology and for the preservation of animal, human and higher plant germplasm but its application for the conservation of algae and cyanobacteria is less widespread. The review provides a theoretical introduction to vitrification and highlights current and potential applications in algal preservation, especially for storage-recalcitrant organisms.
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Document Type: Research Article

Publication date: 01 August 2004

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  • Nova Hedwigia is an international journal publishing original, peer-reviewed papers on current issues of taxonomy, morphology, ultrastructure and ecology of all groups of cryptogamic plants, including cyanophytes/cyanobacteria and fungi. The half-tone plates in Nova Hedwigia are known for their high quality, which makes them especially suitable for the reproduction of photomicrographs and scanning and transmission electron micrographs.
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