Skip to main content

Atmospheric Compensation in Fourier Transform Infrared (FT-IR) Spectra of Clinical Samples

Buy Article:

$29.00 plus tax (Refund Policy)


A new method is proposed for the elimination of the spectral contribution of two atmospheric gases (CO2 and H2O) in Fourier transform infrared (FT-IR) spectra of clinical samples. The algorithm is based on the measurement of reference spectra of H2O followed by an automatic calculation of the spectral contribution of the above-mentioned gases to the sample spectra. Then this contribution is compensated by spectral subtraction. Attenuated total reflectance FT-IR spectra of serum and urine samples in the presence of atmospheric gases were corrected and compared with spectra obtained with an N2 purge. Visual inspection of the spectra as well as calculated noise levels confirmed that the method compensated for the contribution of CO2 and H2O to serum and urine spectra to a great extent. As compared with the correction performed by a commercial software package, the proposed method clearly improved the correction of CO2 and provided similar results for H2O compensation. Correlation values between corrected spectra and those obtained under N2-purged conditions in the fingerprint region were higher than 0.995, and noise values in regions where samples did not absorb confirmed the suitability of the correction. The proposed method is fast, easy, and extensible to other gaseous interferences, devices, and acquisition conditions.

Keywords: Atmospheric compensation; Chemometrics; Human serum analysis; Human urine analysis; Infrared (IR) spectroscopy

Document Type: Research Article


Affiliations: Analytical Chemistry Department, University of Valencia, Edifici Jeroni Muñnoz, 46100, Burjassot, Spain

Publication date: November 1, 2013

More about this publication?

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more