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High-Throughput, High-Resolution Echelle Deep-UV Raman Spectrometer

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We constructed an ultrahigh-throughput, high-resolution ultraviolet (UV) Raman spectrograph that utilizes a high-efficiency filter-stage monochromator and a high-dispersion Echelle spectrograph. The spectrograph utilizes a total of six mirrors and two gratings, with an overall efficiency at 229 nm of ∼18%. The limiting resolution of our spectrometer is 0.6 cm−1 full width half-maximum (FWHM), as measured for 229 nm Rayleigh scattering. Use of a 1 mm‐wide entrance slit gives rise to an approximately 10 cm−1 FWHM resolution at 229 nm. The ultrahigh spectrograph throughput enables ultrahigh signal-to-noise ratio, deep UV Raman spectra that allow us to monitor <1% changes in peptide bond composition. The throughput is measured to be 35-fold greater than conventional deep UV Raman spectrometers.

Keywords: Biomolecules; Echelle; High-resolution; High-throughput; Peptide; Protein; UV Raman spectrometer

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/12-06960

Affiliations: Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA

Publication date: August 1, 2013

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