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Open Access Fourier Transform Infrared Spectrochemical Imaging: Review of Design and Applications with a Focal Plane Array and Multiple Beam Synchrotron Radiation Source

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The beamline design, microscope specifications, and initial results from the new mid-infrared beamline (IRENI) are reviewed. Synchrotron-based spectrochemical imaging, as recently implemented at the Synchrotron Radiation Center in Stoughton, Wisconsin, demonstrates the new capability to achieve diffraction limited chemical imaging across the entire mid-infrared region, simultaneously, with high signal-to-noise ratio. IRENI extracts a large swath of radiation (320 hor. × 25 vert. mrads2) to homogeneously illuminate a commercial infrared (IR) microscope equipped with an IR focal plane array (FPA) detector. Wide-field images are collected, in contrast to single-pixel imaging from the confocal geometry with raster scanning, commonly used at most synchrotron beamlines. IRENI rapidly generates high quality, high spatial resolution data. The relevant advantages (spatial oversampling, speed, sensitivity, and signal-to-noise ratio) are discussed in detail and demonstrated with examples from a variety of disciplines, including formalin-fixed and flash-frozen tissue samples, live cells, fixed cells, paint cross-sections, polymer fibers, and novel nanomaterials. The impact of Mie scattering corrections on this high quality data is shown, and first results with a grazing angle objective are presented, along with future enhancements and plans for implementation of similar, small-scale instruments.

Keywords: Art conservation; Brain; Cancer histology; Chemical imaging; Cultural heritage; FPA; FT-IR imaging; Focal plane array; Fourier transform infrared spectroscopy; IRENI; Infrared microspectroscopy; Infrared synchrotron radiation; Live algal cell imaging; Mouse embryonic stem cells; Neuron; Retina

Document Type: Research Article


Affiliations: Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53211, USA

Publication date: May 1, 2012

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