Selection of Haploid Maize Kernels from Hybrid Kernels for Plant Breeding Using Near-Infrared Spectroscopy and SIMCA Analysis

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Samples of haploid and hybrid seed from three different maize donor genotypes after maternal haploid induction were used to test the capability of automated near-infrared transmission spectroscopy to individually differentiate haploid from hybrid seeds. Using a two-step chemometric analysis in which the seeds were first classified according to genotype and then the haploid or hybrid status was determined proved to be the most successful approach. This approach allowed 11 of 13 haploid and 25 of 25 hybrid kernels to be correctly identified from a mixture that included seeds of all the genotypes.

Keywords: Corn; Haploid selection; Maize; NIR spectroscopy; Near-infrared spectroscopy; PLS; Partial least squares; SIMCA; Single kernel analysis; Soft independent modeling of class analogy

Document Type: Research Article


Affiliations: 1: Ames Laboratory–USDOE, Iowa State University, Ames, Iowa 50011, USA 2: Iowa State University, Department of Agronomy, Ames, Iowa 50011, USA

Publication date: April 1, 2012

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