If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Recognition of a C‐C Mismatch in a DNA Duplex Using a Fluorescent Small Molecule with Application for “Off‐On” Discrimination of C/G Mutation

$29.00 plus tax (Refund Policy)

Buy Article:


The fluorescent small molecule 2-amino-7-methyl-1,8-naphthyridine (AMND) can selectively bind to a cytosine (C) at a C‐C mismatch in double-stranded DNA (dsDNA). The interactions between AMND and C‐C mismatch-containing dsDNA were investigated by measuring ultraviolet (UV) absorption as a function of temperature to obtain melting curves as well as circular dichroism and fluorescence spectra. Results show that AMND strongly stabilizes C‐C mismatch-containing dsDNA, whereas fully matched duplexes are not stabilized under the same conditions. The fluorescence of AMND was efficiently quenched when it was bound to a C‐C mismatch in dsDNA. Binding constants (K 11), obtained by fluorescence titration, were 1.2 × 105 M−1. Although sensing functions depend on the sequences flanking the mismatch site, the change in AMND fluorescence intensity can be utilized to detect the C‐C mismatch-containing dsDNA. Accordingly, discrimination of the C/G mutation in the model sequence (PGR gene rs1255998) was achieved by visualizing fluorescence of AMND. A probe DNA molecule was designed to contain a C opposite the C/G base in the target DNA, and this probe was used to hybridize the target DNA. The fluorescence of AMND was “on” for a C‐G match, while the fluorescence was “off” for a C‐C mismatch. This assay is simple and does not require DNA labeling.

Keywords: 2-Amino-7-methyl-1,8-naphthyridine; Circular dichroism; C‐C mismatch; Fluorescence spectroscopy; Recognition; Single base mutation

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/11-06410

Affiliations: Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, China

Publication date: February 1, 2012

More about this publication?
Related content



Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more