Linear Discriminant Analysis of Single-Cell Fluorescence Excitation Spectra of Five Phytoplankton Species

$29.00 plus tax (Refund Policy)

Buy Article:

Abstract:

Linear discriminant analysis (LDA) of single-cell fluorescence excitation spectra (λem = 680 nm) for five species of marine phytoplankton was used to determine whether intra-species variation among single cells precluded discrimination among species. Single-cell spectra were recorded in an optical trap with a custom-built spectral fluorometer. For nitrogen (N)-replete cells, separation of all five species (Emiliania huxleyi, a coccolithophore, Thalassiosira pseudonana, a diatom, Dunaliella tertiolecta, a chlorophyte, Amphidinium carterae, a dinoflagellate, and Rhodomonas salina, a cryptophyte) was possible using only a portion of the excitation spectra (570‐610 nm). This wavelength region gave perfect classification of species with a minimum Fisher ratio of 62. For four species (E. huxleyi, T. pseudonana, D. tertiolecta, and A. carterae), variations in fluorescence excitation spectra as cells were starved of N did not impact the classification process adversely within the chosen spectral window. R. salina cells grown with and without N showed significant differences in their fluorescence excitation spectra but could still be classified if a different spectral window (490‐570 nm) was used. Overall, we conclude that intra-species variation among single-cell fluorescence excitation spectra does not preclude discrimination among species.

Keywords: Classification; Excitation spectra; Fluorescence; Identification; Phytoplankton

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/11-06294

Affiliations: 1: Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina 29208, USA 2: Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208, USA 3: Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208, USA

Publication date: January 1, 2012

More about this publication?

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more