Home >> Applied Spectroscopy, Volume 66, Issue 1, Pages 12A-31A and 1-135 (January 2012)

Noninvasive In Vivo Measurement of Natural Moisturizing Factor Content in Stratum Corneum of Human Skin by Attenuated Total Reflection Infrared Spectroscopy

Authors: Takada, Shingo1; Naito, Satoru1; Sonoda, Junko2; Miyauchi, Yuuki1

Source: Applied Spectroscopy, Volume 66, Issue 1, Pages 12A-31A and 1-135 (January 2012) , pp. 26-32(7)

Publisher: Society for Applied Spectroscopy

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Abstract:

Water-soluble components in the outermost layer of human skin are called natural moisturizing factor (NMF). NMF is one of the most important factors of skin moisturizing mechanisms. It is known that individuals with psoriasis, atopic dermatitis, and ichthyosis possess skin with lower-than-normal NMF content. Therefore, a noninvasive method for NMF content measurement of the human skin would be very useful to both dermatologists and cosmetic chemists. We attempted to measure the NMF content by focusing on the infrared wavelengths that are commonly absorbed by most NMF components. In order to estimate the intensity of absorption that is common to NMF components, we simulated the attenuated total reflection (ATR)-infrared (IR) spectrum of human skin by the combination of multiple Gaussian peaks. The spectroscopic signal of NMF linearly correlated with the actual NMF content measured by chromatographic methods. Therefore, we concluded that we can determine the skin surface NMF content from the ATR-IR spectrum of skin. This noninvasive and simple method is useful for studying NMF in a wide variety of skin regions and under various skin conditions.

Keywords: Attenuated total reflection; ATR; Infrared spectroscopy; Natural moisturizing factor; NMF; Skin; Stratum corneum; SC; In vivo measurements; Noninvasive measurements

Document Type: Research article

DOI: http://dx.doi.org/10.1366/11-06276

Affiliations: 1: Tochigi Research Laboratories, Kao Corporation, Akabane 2606, Ichikai-Machi, Haga-Gun, Tochigi 321-3497, Japan 2: Tokyo Research Laboratories, Kao Corporation, Bunka 2-1-3, Sumida-ku, Tokyo 131-8501, Japan

Publication date: 2012-01-01

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