Open Access Fluorescence Correlation Spectroscopy: A Review of Biochemical and Microfluidic Applications

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Abstract:

Over the years fluorescence correlation spectroscopy (FCS) has proven to be a useful technique that has been utilized in several fields of study. Although FCS initially suffered from poor signal-to-noise ratios, the incorporation of confocal microscopy has overcome this drawback and transformed FCS into a sensitive technique with high figures of merit. In addition, tandem methods have evolved to include dual-color cross-correlation, total internal reflection fluorescence correlation, and fluorescence lifetime correlation spectroscopy combined with time-correlated single-photon counting. In this review, we discuss several applications of FSC for biochemical, microfluidic, and cellular investigations.

Keywords: BIOANALYSIS; CONFOCAL MICROSCOPY; FCS; FLUORESCENCE CORRELATION SPECTROSCOPY; HYDRODYNAMICS; MICROFLUIDS

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/10-06224

Affiliations: Department of Chemistry and Biochemistry Texas Tech University Lubbock , Texas 79409, USA

Publication date: April 1, 2011

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