Skip to main content

A Note on Determining the Extent of the Water Raman Peak in Fluorescence Spectroscopy

Buy Article:

$29.00 plus tax (Refund Policy)

Abstract:

A method is proposed for automatically determining the upper and lower boundaries of the Raman scatter peak in fluorescence spectroscopy from empirical data. Accurate peak boundaries are needed to calculate accurate Raman peak areas, used for normalizing fluorescence signals to produce data in units that are comparable between instruments. Comparisons of Raman peak boundaries across nine individual instruments (FluoroMax 3 (FM3) fluorometers from HORIBA Jobin Yvon and Cary Eclipse (CE) fluorometers from Varian Inc.) at the excitation wavelength λex = 350 nm reveal consistent results. At 350 nm excitation, the Raman peak was confined by the emission wavelengths of 382–418 nm, with boundaries determined for the FM3 fluorometers deviating by no more than 0.5 nm and 1.5 nm with respect to the start and end of the peak, and CE fluorometers deviating by up to 1.5 nm and 2 nm, respectively. Peak width was a function of fluorometer type and excitation wavelength. For the FM3 instruments, widths increased from approximately 30 nm at λex = 300 nm to 40 nm at λex = 380 nm, while for the CE instruments, peaks were approximately 5–8 nm narrower. Code for implementing the procedure in MATLAB, which allows for the adjustment of input parameters to compensate for noisy data, is provided in the Supplemental Material (available online).

Keywords: CALIBRATION; FLUORESCENCE SPECTROSCOPY; INSTRUMENTATION; INTENSITY STANDARDIZATION; MATLAB PROGRAM; WATER RAMAN PEAK

Document Type: Short Communication

DOI: http://dx.doi.org/10.1366/10-06136

Affiliations: University of New South Wales, School of Civil and Environmental Engineering, Sydney NSW 2052, Australia; Smithsonian Environmental Research Center, PO Box 28, Edgewater, Maryland 21037, USA

Publication date: February 1, 2011

More about this publication?
sas/sas/2011/00000065/00000002/art00020
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more