@article {Liu:2010:0003-7028:1308,
	author = "Liu, Rui and Taylor, Douglas S. and Matthews, Dennis L. and Chan, James W.",
	title = "Parallel Analysis of Individual Biological Cells Using Multifocal Laser Tweezers Raman Spectroscopy",
	journal = "Applied Spectroscopy",
	volume = "64",
	number = "11",
	year = "2010",
	abstract = "We report on the development and characterization of a multifocal laser tweezers Raman spectroscopy (M-LTRS) technique for parallel Raman spectral acquisition of individual biological cells. Using a 785-nm diode laser and a time-sharing laser trapping scheme, multiple laser foci are generated to optically trap single polystyrene beads and suspension cells in a linear pattern. Raman signals from the trapped objects are simultaneously projected through the slit of a spectrometer and spatially resolved on a charge-coupled device (CCD) detector with minimal signal crosstalk between neighboring cells. By improving the rate of single-cell analysis, M-LTRS is expected to be a valuable method for studying single-cell dynamics of cell populations and for the development of high-throughput Raman based cytometers.",
	pages = "1308-1310",
	url = "http://www.ingentaconnect.com/content/sas/sas/2010/00000064/00000011/art00021",
	doi = "doi:10.1366/000370210793334972",
	keyword = "RAMAN SPECTROSCOPY, OPTICAL TWEEZERS, MULTIFOCAL"
}