Parallel Analysis of Individual Biological Cells Using Multifocal Laser Tweezers Raman Spectroscopy

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Abstract:

We report on the development and characterization of a multifocal laser tweezers Raman spectroscopy (M-LTRS) technique for parallel Raman spectral acquisition of individual biological cells. Using a 785-nm diode laser and a time-sharing laser trapping scheme, multiple laser foci are generated to optically trap single polystyrene beads and suspension cells in a linear pattern. Raman signals from the trapped objects are simultaneously projected through the slit of a spectrometer and spatially resolved on a charge-coupled device (CCD) detector with minimal signal crosstalk between neighboring cells. By improving the rate of single-cell analysis, M-LTRS is expected to be a valuable method for studying single-cell dynamics of cell populations and for the development of high-throughput Raman based cytometers.

Keywords: MULTIFOCAL; OPTICAL TWEEZERS; RAMAN SPECTROSCOPY

Document Type: Short Communication

DOI: http://dx.doi.org/10.1366/000370210793334972

Affiliations: 1: NSF Center for Biophotonics Science and Technology, University of California, Davis, Sacramento, California 95817, USA; Department of Biomedical Engineering, University of California Davis, Davis, California 95616, USA 2: NSF Center for Biophotonics Science and Technology, University of California, Davis, Sacramento, California 95817, USA; Department of Pediatrics, University of California Davis Medical Center, Sacramento, California 95817, USA 3: NSF Center for Biophotonics Science and Technology, University of California, Davis, Sacramento, California 95817, USA; Department of Neurological Surgery, University of California Davis Medical Center, Sacramento, California 95817, USA; Lawrence Livermore National Laboratory, Livermore, California 94550, USA 4: NSF Center for Biophotonics Science and Technology, University of California, Davis, Sacramento, California 95817, USA; Lawrence Livermore National Laboratory, Livermore, California 94550, USA. jwjchan@ucdavis.edu

Publication date: November 1, 2010

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