Molecular Fluorescence Enhancement on Fractal-Like Structures

Authors: Luchowski, Rafal1; Shtoyko, Tanya2; Matveeva, Evgenia3; Sarkar, Pabak3; Borejdo, Julian3; Gryczynski, Zygmunt3; Gryczynski, Ignacy4

Source: Applied Spectroscopy, Volume 64, Issue 6, Pages 158A-174A and 563-689 (June 2010) , pp. 578-583(6)

Publisher: Society for Applied Spectroscopy

Buy & download fulltext article:

OR

Price: $29.00 plus tax (Refund Policy)

Abstract:

In this report we discuss strong fluorescence enhancements on electrochemically grown silver nanostructures examined through fluorescence lifetime imaging microscopy (FLIM). Silver fractal-like nanostructures were deposited on three different substrates: glass, plastic, and silicon. For all of the surfaces the same dye was tested, DyLight 649, deposited in the form of a model immunoassay through excitation from a 635 nm pulsed solid-state laser. The brightness improvement in hot spots exceeded 300 fold, which is about two times higher than was observed previously on similar surfaces. The strongest enhancements correspond to the shortest lifetimes, indicating a strong interaction between excited molecules and silver nanostructures. Additionally, the photostability of the fluorescence dye was dramatically increased in the presence of electrochemically deposited silver nanostructures. The production of silver fractals is easy, very controllable, and can be applied to any surface. We therefore believe that silver fractal-like nanostructures can be used successfully in ultrasensitive assays and fluorophore trace detection.

Keywords: FLUORESCENCE MICROSCOPY; FRACTAL-LIKE STRUCTURES; INDEX HEADINGS: HIGH SENSITIVITY DETECTION; NANOSTRUCTURES; SURFACE PLASMONICS

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/000370210791414317

Affiliations: 1: Center for Commercialization of Fluorescence Technologies, UNTHSC, Fort Worth, Texas 76107; Department of Biophysics, Institute of Physics, Marie Curie-Sklodowska University, 20–031 Lublin, Poland 2: University of Texas at Tyler, Tyler, Texas 75799 3: Center for Commercialization of Fluorescence Technologies, UNTHSC, Fort Worth, Texas 76107 4: Center for Commercialization of Fluorescence Technologies, UNTHSC, Fort Worth, Texas 76107; Department of Cell Biology and Anatomy, UNTHSC, Fort Worth, Texas 76107

Publication date: June 1, 2010

More about this publication?
Related content

Tools

Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content

Text size:

A | A | A | A
Share this item with others: These icons link to social bookmarking sites where readers can share and discover new web pages. print icon Print this page