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Open Access Micro- and Macro-Attenuated Total Reflection Fourier Transform Infrared Spectroscopic Imaging

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Abstract:

Fourier transform infrared (FT-IR) spectroscopic imaging has become a very powerful method in chemical analysis. In this review paper we describe a variety of opportunities for obtaining FT-IR images using the attenuated total reflection (ATR) approach and provide an overview of fundamental aspects, accessories, and applications in both micro- and macro-ATR imaging modes. The advantages and versatility of both ATR imaging modes are discussed and the spatial resolution of micro-ATR imaging is demonstrated. Micro-ATR imaging has opened up many new areas of study that were previously precluded by inadequate spatial resolution (polymer blends, pharmaceutical tablets, cross-sections of blood vessels or hair, surface of skin, single live cells, cancerous tissues). Recent applications of ATR imaging in polymer research, biomedical and forensic sciences, objects of cultural heritage, and other complex materials are outlined. The latest advances include obtaining spatially resolved chemical images from different depths within a sample, and surface-enhanced images for macro-ATR imaging have also been presented. Macro-ATR imaging is a valuable approach for high-throughput analysis of materials under controlled environments. Opportunities exist for chemical imaging of dynamic aqueous systems, such as dissolution, diffusion, microfluidics, or imaging of dynamic processes in live cells.

Keywords: ATR-FT-IR IMAGING; ATTENUATED TOTAL REFLECTION; CANCER CELLS; DEPTH OF PENETRATION; DEPTH PROFILING; FINGERPRINTS; FOCAL PLANE ARRAY; FOURIER TRANSFORM INFRARED SPECTROSCOPY; HAIR; INFRARED SPECTROSCOPY; NEAR-INFRARED SPECTROSCOPY; NIR; RAMAN SPECTROSCOPY; REVIEW; SKIN; SPATIAL RESOLUTION; SURFACE-ENHANCED ATR IMAGING; TISSUE; TOTAL INTERNAL REFLECTION

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/000370210791211673

Affiliations: Department of Chemical Engineering, Imperial College London, SW7 2AZ, London, England

Publication date: May 1, 2010

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