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A Comparison of Pseudomonas aeruginosa Biofilm Development on ZnSe and TiO2 Using Attenuated Total Reflection Fourier Transform Infrared Spectroscopy

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Abstract:

The growth of Pseudomonas aeruginosa PAO1 biofilms on ZnSe internal reflection elements (IREs) was compared with their growth on TiO2-coated ZnSe over several days using attenuated total reflection Fourier transform infrared (ATR-FT-IR) spectroscopy. The effect of the TiO2 coating on the IR spectra of reference compounds and cell suspensions was determined to aid in the interpretation of the data. The presence of TiO2 on the surface of a ZnSe IRE tripled the size of the amide II peak and facilitated the detection of pyoverdin production due to its increased adsorption on the coated surface. A 50% increase in the length of the lag phase was observed for PAO1 growth on TiO2-coated surfaces as compared to growth on ZnSe. Biofilms on both surfaces exhibited a growth maximum for all components, followed by restructuring at the surface characterized by a decrease in the signal. The composition of biofilms grown on TiO2 was relatively constant after the restructuring phase, while the extracellular polymeric substance (EPS) component of the biofilms grown on ZnSe gradually increased. The peak due to the carbohydrate component of EPS was much larger in the spectra of biofilms than in those of planktonic cells. The increase of the pyoverdin signal over time in the spectra of the biofilms on TiO2 closely followed the overall increase in biomass. However, no signal from pyoverdin was detected in the presence of ferric ions.

Keywords: ATR; ATTENUATED TOTAL REFLECTION; BIOFILM; FOURIER TRANSFORM INFRARED SPECTROSCOPY; FT-IR SPECTROSCOPY; PSEUDOMONAS AERUGINOSA PAO1; PYOVERDIN

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/000370209789379259

Affiliations: 1: Department of Chemistry, St. Francis Xavier University, Antigonish, N.S., Canada B2G 2W5 2: Department of Human Nutrition, St. Francis Xavier University, Antigonish, N.S., Canada B2G 2W5 3: Department of Physics, St. Francis Xavier University, Antigonish, N.S., Canada B2G 2W5; Centre for Applied Petroleum Sciences, St. Francis Xavier University, Antigonish, N.S., Canada B2G 2W5; Advanced Foods and Materials Network – Network of Centres of Excellence (AFMNet-NCE), 150 Research Lane, Suite 215, Guelph, Ontario, Canada, N1G 4T2 4: Department of Chemistry, St. Francis Xavier University, Antigonish, N.S., Canada B2G 2W5; Centre for Applied Petroleum Sciences, St. Francis Xavier University, Antigonish, N.S., Canada B2G 2W5

Publication date: September 1, 2009

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